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Immunohistochemical analysis confirmed that OSR downregulated inflammatory mediators and oxidative stress markers while upregulating GPX4 and SLC7A11 expression in joint tissues.Furthermore, OSR demonstrated excellent systemic tolerability, with consistent body weight and preserved histoarchitecture across major organs, indicating minimal offtarget toxicity 32,33 .Current pharmacological treatments for OA primarily provide symptomatic relief rather than disease modification.While NSAIDs and analgesics provide transient pain relief, they are ineffective in rebuilding cartilage or interrupting the chronic inflammatory and oxidative feedback loops that propel osteoarthritic progression 34

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Breast feeding women require 2.0 g although pregnant women are not believed to require any additional B12

A significant difference between groups was determined using one-way ANOVA followed by the post hoc Tukey test, with a P-value less than or equal to 0.05 considered significant for all statistical analyses