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The mice in the HFHC group were fed a high-trans-fat diet [58 kcal% fat w/sucrose Surwit Diet (D12331, lot number: 17082101A10, Research Diets, USA)] combined with water containing high carbohydrates (55% fructose + 45% sucrose, concentration 42 g/L) to establish an animal model of NASH

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(2) Prefrontal Cortex Parallel analysis in the prefrontal cortex revealed consistent patterns of microglial activation (Fig