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Notably, LMF-induced calcium influx through voltage-gated channels may act as a trigger for EV biogenesis and cargo sorting, as calcium signaling is known to regulate vesicle trafficking and the matrix metalloproteinase (MMP) activation [59-61] [Figure 1]

10.1007/s00726-011-1206-6 Summary Keywords Pichia pastoris, peptide-crossing-blood-brain-barrier, delta-sleepinducing peptide, neurotransmitter balance, mouse model, PCPA-induced insomnia Citation Mu X, Qu L, Yin L, Wang L, Liu X and Liu D (2024) Pichia pastoris secreted peptides crossing the blood-brain barrier and DSIP fusion peptide efficacy in PCPA-induced insomnia mouse models