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121 Mutations in the progranulin gene, GRN , which cause a genetic familial FTD, downregulate expression of EAAT2 astrocyte glutamate transporter, which mediates synaptic degeneration

The LC treated monolayers of VSMCs in 25-cm 2 culture flasks were rinsed twice with 1 mmol/L EDTA in PBS, lysed with Triton lysis buffer (50 mmol/L TrisHCl, pH 8.0, containing 150 mmol/L NaCl, 1% Triton X-100, 0.02% sodium azide, 10 mmol/L EDTA, 10 mg/mL aprotinin and 1 mg/mL aminoethylbenzenesulfonyl fluoride) and then saved after centrifugation

Keep sealed tightly

Proper vial handling technique is essential for maintaining sterility throughout the use period

This proactive approach can help avoid confusion and ensure that any not-negative result is appropriately handled