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After fixation, the cells were washed with PBS and permeabilized with 0.1% Triton X-100 (Daejung 8566-4405) in PBS for 10 min at room temperature

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| Protocol Design | MOTS-c Dosing | 5-Amino-1MQ Dosing | Timing Strategy | Cycle Length | Best Suited For | Professional Assessment | ||||||| | Standard Metabolic Stack | 10mg subcutaneous, 3x/week (Mon/Wed/Fri) | 100mg oral, daily | MOTS-c AM fasted

The animals in groups 3 to 6 were taken as drug treatment groups i.e