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Lentiviruses were generated by co-transfection of each shRNA construct with envelope plus packaging vectors (pMD2.G, Addgene #12260 and psPAX2, Addgene #12259) and viral supernatants purified by ultracentrifugation onto 20% sucrose gradients

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This post will explore whether a 500 mg dose is safe, how your body uses this tripeptide, and why the delivery method matters just as much as the milligram count