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179,180 High-salt diet HSD has long been considered as a risk factor and trigger of malignancies

10.1016/S1522-5720(02)00064-8 Clin Fam Pract

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ROS/PI3K/Akt and Wnt/-catenin signalings activate HIF-1-induced metabolic reprogramming to impart 5-fluorouracil resistance in colorectal cancer

Cells were then blocked with 5% goat serum for 1 h at R.T and stained with 1300 dilution of anti-GST A4-4 polyclonal antibody overnight at 4C, followed by incubation with FITC-conjugated secondary antibody for 1 h at room temperature as described before [29]