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The combined effects of HGH levels and IGF-1 contribute to the hypertrophy (increase in muscle size) and hyperplasia (increase in muscle cell number) of skeletal muscles, ultimately resulting in enhanced muscle mass

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p53 TAD (13-61) was subcloned into the pET His 6 glutathione-S-transferase (GST) TEV LIC cloning vector (2G-T, obtained from Scotta Gradia, Addgene plasmid #29707) and transformed into BL21 (DE3) pLysS cells