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2.2 Construction of cDNA cDNA encoding the following constructs has been previously described: C-terminally labeled super yellow fluorescent protein 2 (SYFP2) and Nano Luciferase (NLuc)-labeled human GLP-1R and GIPR, arrestin3NLuc, arrestin3SYFP2 (Arr3SYFP2), and mCherry CAAX ( vice versa (Table 1)

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Cell-based assays and in vitro systems are used by scientists to study the binding properties, structural stability, and signaling activity under controlled conditions