RSMC mutants were generated by PCR: RSMCSIM (RSMC46-6099-112-10A, 10 A = R30A, R31A, R33A, R35A, S65A, E87A, E89A, S98A, D113A, E115A), and RSMC-16A with additional lysine-to-alanine substitutions (K52A, K53A, K103A, R104A, K106A, K107A) introduced into the RSMC10A background
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