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A protein was considered for quantification if it presented at least 2 identified peptides and if there was an intensity value in at least 75% of the replicates in one of the two groups

Men experience a more rapid loss between the ages of 41 and 60, whereas women experience the rapid loss after the age of 60, which is to do with protective hormone levels up until that point

Cell debris was removed by centrifugation at 4,400 g at 4 C for 30 min, and the clarified cell lysate was directly used for biocatalytic reactions

A common research-context setup includes the 80 mg KLOW vial(s), one 10 mL bottle of bacteriostatic water per 1-2 vials, U-100 insulin syringes (one per daily injection 30- or 50-unit syringes are easier to read for small draws), and alcohol prep pads (two per injection)

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