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The formaldehyde-containing DMEM was removed and cells were washed 3 times with 20ml of ice-cold PBS supplemented with 1x complete protease inhibitor cocktail

The total number randomized, and gender distribution were not consistently reported

Conclusion Melanotan 2 is a tanning peptide that bodybuilders have long understood the benefits of, and those in bodybuilding circles have used it for years in the build-up to competitions

The cells were then washed three times with PBS and incubated with Oil Red O working solution (G1260, Solarbio) for 30 min, protected from light

Age-dependent upregulation of p53 and cytochrome c release and susceptibility to apoptosis in skeletal muscle fiber of aged rats: role of carnitine and lipoic acid