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Relationship between RS, Aging, and Related Diseases Reactive oxygen, nitrogen, and halogen species, collectively referred to as reactive species (RS), are produced through various internal and external processes

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Although no randomized trials exist, the uniformity of observational data has led GeneReviews and European neurometabolic taskforces to issue class I recommendations for immediate high-dose riboflavin upon molecular diagnosis (14)

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Cell culture HOXC8-imESCs, Control-imESCs, and parental cell line imESCs were maintained by 10 cm collagen-coated culture dishes (Corning Life Science, Tewksbury, MA) in DMEM/F12 medium (Wako, Osaka, Japan), supplemented with 10% FBS (Gibco, Rockville, MD), 2 mM L-glutamine (Gibco), and a 1% antibiotic-antimycotic solution (Wako), at 37 C in a humidified atmosphere of 5% CO and 95% air