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derivatives may be effective at lower concentrations) (optimal ~15% (for pure ascorbic acid

Use extra caution if you have a history of pancreatitis, gallbladder disease, severe gastrointestinal disease, or diabetic retinopathy

Immunoblot analysis Whole cell lysates were prepared using radio-immunoprecipitation assay (RIPA) lysis buffer (pH 7.8) containing 25 mM Tris-HCl, 120 mM NaCl, 5 mM EDTA and 0.5% NP-40 supplemented with 100 g/mL phenylcmethylsulfonyl fluoride, 200 mM sodium orthovanadate, 0.5 M NaF, protease inhibitor cocktail sets III and IV and phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich)

Step 4 - The fourth step of the non-oxidative phase is the final step

This scarcity of intracellular Gln hampers the synthesis of asparagine and proteins encoded by the mitochondrial genome, ultimately leading to ROS-induced apoptosis in tumor cells (45)