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Choosing your reconstitution volume Because water volume is the free variable, the choice comes down to a trade-off between resolution and vial capacity

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Each trial involved placing the animal on a rotarod revolving at 5 rpm/min, then accelerating at 0.6 rpm/min

If you are using a different BAC water volume (e.g., 1ml instead of 2ml), the formula adjusts accordingly: Injection volume (ml) = Desired dose (mg) / Concentration (mg/ml) Concentration (mg/ml) = Vial size (mg) / BAC water added (ml) The recommended starting approach for the Glow Stack is to begin at the lower end of the dose range and assess tolerance before titrating upward