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For the calibration standard and quality control samples, 5 l aliquot of each CS and QC solutions was combined with 95 l of blank plasma, vortexed for 30 s before 50 l of IS working solution (dexketoprofen, 1 g/ml) and 350 l of methanol were added to the plasma samples, which were mixed via vortex for 1 min and centrifuged at 20,200 g for 10 min at 4C