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In a microwell ELISA plate pre-coated with anti-mouse glutathione peroxidase (GSH-Px) antibody (solid phase antibody), add mouse glutathione peroxidase (GSH-Px) calibrator and the sample to be tested, then add biotin-labeled antibody, incubate and fully wash, then add HRP-coupled avidin, incubate and fully wash to remove unbound components, and form a sandwich complex of solid phase antibody-antigen-biotin-labeled antibody-avidin enzyme on the solid surface of the microwell plate

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