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While DNA vaccines show great promise in preclinical studies, challenges remain in optimizing delivery methods and ensuring long-term efficacy, particularly when translating findings from animal models to human clinical trials

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Adipose tissue in obesity releases proinflammatory cytokines such as tumor necrosis factor (TNF), interleukin6 (IL6) and monocyte chemoattractant protein1 (MCP1), while Kupffer cells and infiltrating macrophages in the liver amplify this inflammatory milieu, contributing to hepatocellular injury and fibrosis [26, 27]