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Characterization of the estradiol-binding site structure of human pancreas-specific protein disulfide isomerase: Indispensable role of the hydrogen bond between His278 and the estradiol 3-hydroxyl group

Fragment ions generated during analysis reveal the amino acid arrangement

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After 12 hours of thermal stress, 6 fish were randomly sampled from each tank, anesthetized with MS-222 (0.125 g/L), and dissected to collect liver tissues, which were then frozen in liquid nitrogen for subsequent analysis