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When B12 runs low, symptoms tend to creep in slowly enough to get blamed on something else: persistent fatigue, brain fog, low mood, weight gain or difficulty losing weight, weakness, tingling in the hands or feet, shortness of breath, and a weakened immune system

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Blots were blocked with phosphate buffered saline with 0.1% Tween 20 and then binding with antibodies for eNOS (1: 1000) or caveolin-1 (1: 1000), followed by horseradish peroxidase-conjugated goat anti-rabbit (diluted 1: 5000) and enhanced chemiluminescence reaction was performed for blots development

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