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Besides rennet enzymes (chimosin, pepsin), non-dairy related proteases, including plant cardosin, bromelain, papain, actinidain, ficin, fungal flavorzyme, and bacterial subtilisin, have also been used to obtain functional foods [30,31] or to produce hydrolysates enriched in antimicrobial and antioxidant peptides

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Serum homogenate was again centrifuged at 21,000 g for 5 minutes at 4 C after which the supernatant was pooled with the previously isolated metabolite fraction