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19 Immunohistochemistry For paraformaldehyde (PFA) fixation, mice were deeply anesthetized with isoflurane and perfused sequentially with heparinized normal saline, followed by 4% PFA and 0.5% glutaraldehyde

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Consistently, the number of tumors in the colon of Apc +/Min /Stard7 IEC mice increased no matter what their grade was (lower, mild or high grades) (Fig

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