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Retrieval of extracellular RNA and cDNA synthesis We ultracentrifuged cell-free urine and serum samples at 100,000 g for 2 h at 4 C, removed the supernatant, and extracted RNA from the translucent ribonucleoprotein pellet using 1 ml TRIzol Reagent according to the manufacturers instructions

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The breakage of the -glutamyl bond catalyzed by GGT can alter the dispersed state of the probe and trigger both the AIE and ESIPT effects