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Similarly, substituting the ethoxy group at position R 2 with the smaller methoxy ( 28 ) or hydroxy ( 29 ) groups did not hinder solubility but reduced binding to TRF1 TRFH in the CPMG assay

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doi:10.1074/jbc.270.16.9543

Nasal spray considerations: Typically lower bioavailability than injection, requiring somewhat higher doses Faster onset in some users due to direct CNS access More convenient and less intimidating for needle-averse individuals Requires properly calibrated spray bottles for accurate dosing Converting from injectable to nasal dosing is not straightforward