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For immunogold labeling analysis, high-pressure frozen root tips were transferred to the AFS (Leica Microsystems) and freeze-substituted in dry acetone containing 0.1% uranyl acetate at 85 C

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Among other cells, our immune system also consists of mast cells, which can release a wide variety of chemical mediators such as: Histamine Interleukins Prostaglandins Cytokines Chemokines When you are exposed to an allergen, toxin, and/or injury, these chemical mediators, especially histamine can be released into your body