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In the absence of efficient repair mechanisms, cell membranes in yeast cells are oxidized, the structure and function of proteins are modified, and DNA is damaged [16]

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So I'm certainly not saying anything negative about anyone

the gel purified digested His6-AV-Ubiquitin- semapeptide (nucleotide sequence) encoding gene PCR product was ligated into Ndel and Xhol sites of pET24 plasmid vector using Quick ligation kit (NEB)