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Determination of TGR5 and PLC- by western blot Lysates were prepared from STC-1 cells and were separated by SDS-PAGE followed by transfer onto nitrocellulose membranes (Immobilon-FL, Millipore, Billerica, MA)

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Mitochondrial membrane damage during aging process in rat heart: potential efficacy of L-carnitine and DL alpha lipoic acid