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Following NEM or DMSO treatment, samples were incubated with a 2.3 M mixture of three activity-based probes: Biotin-Ahx-Ub-VME (UbiQ-054), Biotin-Ahx-Ub-PA (UbiQ-076), and Biotin-Ahx-Ub-VS (UbiQ-188), for 60 minutes at 300 rpm and 25 C

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Researchers across multiple disciplines examine peptides to gain insights into biological processes, signaling pathways, and other scientific areas of interest

In the present study, PHH not only indicated an upregulation of glutathione upon NFT expose but also confirmed an elevated resilience of NFT pre-treated cells toward other stressors