A supplementation during prenatal ethanol exposure alters the expression of fetal rat liver genes involved in oxidative stress regulation
Our expert team is with you every step of the way, offering science-backed support through secure virtual visits
Evaluation of antioxidant capacity of SAC DPPH Free Radical Scavenging Capacity Assay Kit (Nanjing Jiancheng Bioengineering Institute, A153-1-1, Nanjing, China): Prepare the working solution according to the kit instructions, mix with different concentrations of SAC solution, incubate in the dark at 25 for 30 min, centrifuge at 4000 rpm for 5 min, transfer 100L to a cuvette, zero with 80% methanol, and measure absorbance at 517 nm
Recommendations vary widely and there is no clear consensus
The advanced protocol should only be implemented after successful tolerance of lower doses and under appropriate medical supervision