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Cells were washed with PBS to remove excessive NEM before lysing in triton lysis buffer (50 mM TrisHCl, pH 7.5, 1 mM EDTA, 1% (v/v) Triton X-100, 150 mM NaCl) supplemented with protease inhibitor cocktail (EMD Millipore, 539134)

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Despite the differences among theories, current research agrees that epigenetic alterations and hormonal dysregulation, leading to inflammation, are central features of endometriosis (12), with reactive oxygen species (ROS) serving a key role in this inflammatory response (4)

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However, injection is not without drawbacks