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The iron response mediated by transferrin receptor (TFR) plays a key role in doxorubicin (DOX)-induced apoptosis 27

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PCR amplification involved 30 cycles (94 C for 40s, 60 C for 40s, 72 C for 40s) after an initial 95 C/5min denaturation, followed by a final 72 C/5min extension, with products visualized on a 1% agarose gel and the recombinant vector subsequently digested with ECOR 1 and Xba 1 for sequencing (GENEray, China)