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Cells cultured in 12-well plates were incubated for 30 min with fluorescein-labelled human insulin, then washed to remove unbound material, and observed using fluorescence microscopy

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While BCAAs oxidation is thought to be nonessential for cardiac energy production 73 , BCAA metabolism may play a pivotal role in the regulation of cardiac substrate metabolism

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Moreover, short-term experiments may not be able to detect the impact of ANG II suppression on aldosterone levels, since aldosterone has slower rates of secretion and degradation than ANG II (66)