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Samples then were eluted with elution buffer [1% (w/v) SDS, 250 mM NaCl, 1 mM EDTA, 10 mM TRIS pH 8] for 10 minutes at 65C, and analyzed using SDS-PAGE on an 8% or 12% acrylamide gel

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1 Introduction Posttraumatic stress disorder (PTSD) is a stress-associated complex and chronic mental disorder that develops following direct or indirect exposure to an extremely stressful (traumatic) event or series of events (Ayano et al., 2020)